Fig. 5

Src overexpression alters cell shape of plectin knockout melanoma cells. A Src was overexpressed in B16 and PKO1. Expression and phosphorylation of PYK2 was determined by western blotting analysis. B Cells were immunostained with anti-Flag/Alexa fluor 488, Rhodamine-phalloidin and DAPI 1 d after Src introduction. Cell shapes are outlined with a dotted line in the bright-field image. Scale bar = 50 μm. C The number of elongated cells were quantified (n = 5). *; p < 0.05 vs control Src(−), #; p < 0.05 vs PKO1 Src(−), ns; no significance vs control Src(−)