Fig. 7

MiR-659-3p inhibits osteosarcoma tumor growth and metastasis in vivo. A Tumor growth curve in nude mice indicated that miR-659-3p inhibited tumor growth (red line) compared to scramble miRNA treatment (blue line) (n = 6, P < 0.001). B Picture of tumors at the end time point showed smaller sizes of miR-659-3p treated tumors compared to scramble miRNA treated. C Representative histology of primary tumor from scramble miRNA injected mouse. D Representative histology of primary tumor from miR-659-3p mimic injected mouse. E Representative histology of lung metastasis tumor from scramble miRNA injected mouse. F Representative histology of lung metastasis tumor from miR-659-3p mimic injected mouse. G Quantification of lung metastasis from the lungs (n = 6, P < 0.001). H. Real-Time PCR showed SRPK1 expression on mRNA level was downregulated in miR-659-3p mimic treated tumors compared to scramble miRNA treated tumors (n = 6). I Western blot showed protein expression in the tumor tissue treated with miR-659-3p mimic compared to scramble miRNA treated. All western blots were performed on different gels. J Representative image of DAPI staining of primary tumor from miR-659-3p mimic injected mouse. K Representative image of SRPK1 immunostaining of primary tumor from scramble miRNA injected mouse. L Representative image of DAPI staining of primary tumor from scramble miRNA injected mouse. M Representative image of SRPK1 immunostaining of primary tumor from miR-659-3p mimic injected mouse. *** indicates P < 0.001 in the indicated comparison