Fig. 3

Inhibiting miR-659-3p increases osteosarcoma cells growth, migration, and invasion. A Transfection of miR-659-3p inhibitor decreased miR-659-3p level in Saos-2 osteosarcoma cells. B Real-time PCR revealed that inhibiting miR-659-3p increased SRPK1 mRNA expression by 3.7 folds in Saos-2 cells. Transfection of Has-miR-1225-5P inhibitor increase U2OS cells growth. C Western blot confirmed that SRPK1 expression was up-regulated on protein level by inhibiting miR-659-3p. SRPK1 and GAPDH western blots were performed on different gels. D Inhibiting miR-659-3p promoted Saos-2 cell growth. E Representative pictures of Saos-2 cells wound gap at 0, 12, and 24 h of culture. F Representative pictures of crystal violet stained Saos-2 cells invaded through transwells to the bottom chamber of the wells. G Quantification of Saos-2 cells wound gap after 12 and 24 h (n = 3), which showed miR-659-3p inhibitor significantly increased wound healing in cell culture dishes. H. Quantification of Saos-2 cells in the bottom chambers (n = 3). ** indicates P < 0.01 and *** indicates P < 0.001 compared to scramble control