Fig. 2

MiR-659-3p inhibits osteosarcoma cells growth, migration, and invasion. A Real-time PCR confirmed that transfection of miR-659-3p mimic increased miR-659-3p in osteosarcoma cells. B Real-time PCR showed downregulated SRPK1 expression by miR-659-3p mimic transfection. C Western blot confirmed SRPK1 expression was down-regulated on protein level. SRPK1 and GAPDH western blots were performed on different gels. D Transfection of miR-659-3p inhibited HOS cell growth. E Representative pictures of HOS osteosarcoma cells wound gap at 0, 12, and 24 h of culture. F Quantification of the HOS cell wound gap after 12 and 24 h culture (n = 3), which showed transfection of miR-659-3p mimic slowed HOS wound healing in cell culture dish. G Representative pictures of crystal violet stained HOS cells in the bottom chamber of transwells. H Quantification of HOS cells migrated through transwells to the bottom chamber (n = 3). ** indicates P < 0.01 and *** indicates P < 0.001 compared to Scramble control