Fig. 5

hsa-miR-1248 directly binds with PSMD10 and regulates its expression in CRC cells. A Results of Droplet digital polymerase chain reaction (ddPCR) assay showing the mRNA expression of PSMD10 in the Normal, M_low, and M_High tissues; each group contained three samples. B Sequences of Hsa-miR-1248, PSMD10-WT, and PSMD10-Mut; (C) Results of dual-luciferase assay showing the relative luciferase activity in SW480 cells transfected either with PSMD10-WT or PSMD10-Mut. D Results of dual-luciferase assay showing the relative luciferase activity in SW620 cells transfected either with PSMD10-WT or PSMD10-Mut; (E) Results of quantitative real-time polymerase chain reaction showing the expression of PSMD10 in SW480 cells transfected with anti-miR-1248 or anti-miR-NC. F Expression of PSMD10 in SW620 cells transfected with miR-1248 or miR-NC; (G) Western blots showing the expression of PSMD10; α-tubulin served as the loading control (The edges of the blots are cropping, and the full-length blots are presented in Supplementary Fig. 1). The results in (C-F) are presented as mean ± standard deviation of three independent experiments. *P < 0.05