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Fig. 1 | BMC Cancer

Fig. 1

From: Flagellin synergistically enhances anti-tumor effect of EGFRvIII peptide in a glioblastoma-bearing mouse brain tumor model

Fig. 1

Characterization of EGFRvIII-GL261/Fluc cells. a Both GL261/Fluc and EGFRvIII-GL261 cells were double-stained with WGA (wheat Germ Agglutinin, red) and EFGRvIII (green). Nuclei were stained with DAPI (blue). b EGFRvIII expression on the cell surface was detected with Alex488 by flow cytometry. c EGFRvIII- GL261/Fluc cells were treated without (-) or with ( +) LY294002 (PI3K inhibitor) to identify the kinetic activity of EGFRvIII. Phosphorylation levels of PI3K and AKT from cell lysates were analyzed by western blotting using p-PI3K p85(Y458) and pAKT(S473) antibodies. Full-length blots/gel are presented in Supplementary Fig. 1

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BMC Cancer

ISSN: 1471-2407

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