Table 2 Summary of the analyzed m6A proteins as indicated and their correlation with overall survival (indicated as %alive) for the entire study cohort, HPV-independent, and HPV-dependent VSCC. The HPV-status was not available for 24 patients. Samples were grouped according to high and low expression based on the staining intensities. p-values for the group comparisons are based on log-rank tests (significance threshold p < 0.5). q-values are based on multiple hypotheses testing using the method of Benjamini and Hochberg with a significance threshold of q < 0.1
All | HPV-independent | HPV-dependent | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Proteins | Localisation | Staining intensity (low/high) | N (low/high) | %alive (low/high) | p-value | N (low/high) | %alive (low/high) | p-value | N (low/high) | %alive (low/high) | p-value | q- value |
Writer | ||||||||||||
METTL3 | nuclear | 0–2 + /3 + | 77/27 | 62.3/40.7 | 0.104 | 51/18 | 56.9/38.9 | 0.435 | 11/7 | 81.8/28.6 | 0.010 | 0.08 |
METTL4 | cytoplasmatic | 0–1 + /2–3 + | 60/36 | 58.3/50.0 | 0.113 | 44/24 | 54.5/45.8 | 0.128 | 8/7 | 87.5/42.9 | 0.034 | 0.10 |
METTL14 | nuclear | 0–1 + /2–3 + | 54/45 | 55.6/55.6 | 0.586 | 44/23 | 47.7/60.9 | 0.541 | 6/10 | 100/55.6 | 0.020 | 0.09 |
WTAP | nuclear | 0–2 + /3 + | 90/16 | 54.4/68.8 | 0.311 | 63/6 | 49.2/83.3 | 0.132 | 16/4 | 68.8/75.0 | 0.694 | 0.74 |
KIAA1429 | nuclear | 0–1 + /2–3 + | 66/35 | 56.1/60.0 | 0.782 | 47/22 | 53.2/59.1 | 0.661 | 10/5 | 60.0/40.0 | 0.482 | 0.70 |
Eraser | ||||||||||||
FTO | nuclear | 0–2 + /3 + | 83/21 | 55.4/76.2 | 0.113 | 61/9 | 54.1/66.7 | 0.361 | 10/9 | 50.0/77.8 | 0.257 | 0.48 |
ALKBH5 | cytoplasmatic/ nuclear | 0–1 + /2–3 + | 44/63 | 50.0/65.1 | 0.231 | 35/35 | 45.7/62.9 | 0.161 | 4/15 | 50.0/66.7 | 0.699 | 0.74 |
Reader | ||||||||||||
HNRNPA2B1 | nuclear | 0–2 + /3 + | 85/23 | 57.6/73.9 | 0.429 | 62/10 | 54.8/70.0 | 0.602 | 17/2 | 58.8/100 | 0.297 | 0.48 |
HNRNPC | nuclear | 0–1 + /2–3 + | 54/48 | 51.9/62.5 | 0.162 | 34/35 | 50.0/54.3 | 0.518 | 11/7 | 63.6/71.4 | 0.736 | 0.74 |
YTHDC1 | membraneous/ cytoplasmatic/ nuclear | 0–2 + /3 + | 78/23 | 61.5/43.5 | 0.111 | 58/12 | 58.6/41.7 | 0.496 | 12/7 | 83.3/28.6 | 0.012 | 0.08 |
YTHDF1 | cytoplasmatic | 0–2 + /3 + | 70/26 | 57.1/50.0 | 0.893 | 52/15 | 55.8/33.3 | 0,409 | 10/4 | 60.0/75.0 | 0.551 | 0.72 |
YTHDF2 | cytoplasmatic | 0–1 + /2–3 + | 31/73 | 58.1/54.8 | 0.422 | 21/48 | 52.4/52.1 | 0.665 | 5/13 | 100/46.2 | 0.040 | 0.10 |
YTHDF3 | cytoplasmatic | 0–1 + /2–3 + | 45/51 | 55.6/58.8 | 0.725 | 28/37 | 50.0/56.8 | 0.554 | 6/7 | 66.7/57.1 | 0.084 | 0.18 |