Fig. 4.

22Rv1 cells were subcutaneously implanted with XIST-shRNA1-infected cells to establish a xenograft model of PCa. (A) Tumour volumes of animals were measured weekly. (B) Representative photographs of xenograft tumors were taken after 4 weeks. (C) Tumour weights in each animal were calculated. (D) qRT‒PCR was used to detect the level of lncRNA XIST in tumour tissues. (E) Knockdown of lncRNA XIST significantly decreased the percentage of Ki-67-positive cells and increased the number of apoptotic cells in the tumours compared with the NC group (200 ×) **P < 0.01 vs. NC group