Fig. 3

Downregulation of lncRNA XIST inhibited proliferation and invasion but induced apoptosis of PCa cells. (A) Vcap cells were transfected with NC or XIST-shRNA1 for 24, 48 and 72 h. A CCK-8 assay was used to measure cell viability. (B) Vcap cells were transfected with NC or XIST-shRNA1 for 72 h. Relative fluorescence expression levels were quantified by Ki67 and DAPI staining. (C) Vcap cells were transfected with NC or XIST-shRNA1 for 24 h. Cell invasion was detected using a transwell invasion assay. (D) Vcap cells were transfected with NC or XIST-shRNA1 for 72 h. Apoptotic cells were detected with Annexin V and PI double staining. (E) The expression levels of Bax, Bcl-2, and active caspase 3 in 22Rv1 cells were detected with western blotting. β-actin was used as an internal control. (F, G, H) The relative expression levels of Bax, Bcl-2, and active caspase 3 in 22Rv1 cells were quantified via normalisation to β-actin. **P < 0.01 vs. NC group