Fig. 1

NOTCH1 protein expression of ATL and HTLV-1-infected T-cell lines. Western blots of NOTCH1-ANK domain antibody (Ab) to mN1A in ATL and HTLV-1-infected T-cell lines. Normal protein expression of full-length NOTCH1 (~ 300 kDa) and NTM (~ 120 kDa) was detected in NOTCH1 non-translocated JURKAT cells. High protein expression of NTM (~ 120 kDa) was found in ATL and HTLV-1-infected T-cell lines, regardless of Tax expression, except Su9T01. Absence of full-length NOTCH1 bands and aberrant protein expression undercut 117 kDa wild-type polypeptide, implying NOTCH1 translocation-driven expression in SUP-T1. Red band is non-specific