Fig. 6

Effects of miR-29b-3p on the NF-κB signaling pathway protein by targeting HuR in AML cells. a, c Immunofluorescence experiment, fluorescent expressions of p65 were observed using fluorescence microscope (×200), images were longitudinally aligned represented as total pattern, nuclear and merge pattern, respectively. b, d Fluorescent expressions of merge pattern dividing by total pattern represented as the relative fluorescence rate of p65. e p65 at nuclear, cytosol and total cellular proteins were detected by western blot. f-k Quantification of nucleus p65 was normalized to PCNA, quantification of cytoplasm and total p65 were normalized to GAPDH. l p65, IκBα and their phosphorylation proteins expression were detected by western blot. The original blots/gels are presented in Supplementary Figure 6. m-p Quantifications of phosphorylated p65 and phosphorylated IκBα were normalized to p65 and IκBα after miR-29b-3p over-expression and inhibition, respectively. (*P<0.05, **P<0.01, vs. NC group). The experiments were repeated at least three times