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Fig. 6 | BMC Cancer

Fig. 6

From: GPX4 suppresses ferroptosis to promote malignant progression of endometrial carcinoma via transcriptional activation by ELK1

Fig. 6

ELK1 binds directly to the GPX4 promoter to regulate the expression of GPX4 in EC cells. A Double luciferase reporter assay confirmed the interaction between ELK1 and GPX4 promoters in Ishikawa cells. B ELK1 mRNA expression in EECs and EC cell lines was analyzed by qRT-PCR. C Correlation between ELK1 and GPX4 in normal endometrial and EC cells in mRNA level was analyzed. D ELK1 protein expression in EECs and EC cell lines was analyzed by western blot. E Correlation between ELK1 and GPX4 in normal endometrial and EC cell lines in protein level was analyzed. F The expression level of GPX4 mRNA was identified by qRT-PCR after ELK1 overexpression. G The expression level of GPX4 mRNA was identified by qRT-PCR after ELK1 down expression. H The binding sites of ELK1 and GPX4 were predicted by the JASPAR database. I ChIP assay verified that ELK1 could bind to GPX4 promoter in Ishikawa cells. J The length of the qPCR product was tested by DNA gel

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