Fig. 6From: GPX4 suppresses ferroptosis to promote malignant progression of endometrial carcinoma via transcriptional activation by ELK1ELK1 binds directly to the GPX4 promoter to regulate the expression of GPX4 in EC cells. AÂ Double luciferase reporter assay confirmed the interaction between ELK1 and GPX4 promoters in Ishikawa cells. BÂ ELK1 mRNA expression in EECs and EC cell lines was analyzed by qRT-PCR. CÂ Correlation between ELK1 and GPX4 in normal endometrial and EC cells in mRNA level was analyzed. DÂ ELK1 protein expression in EECs and EC cell lines was analyzed by western blot. EÂ Correlation between ELK1 and GPX4 in normal endometrial and EC cell lines in protein level was analyzed. FÂ The expression level of GPX4 mRNA was identified by qRT-PCR after ELK1 overexpression. GÂ The expression level of GPX4 mRNA was identified by qRT-PCR after ELK1 down expression. HÂ The binding sites of ELK1 and GPX4 were predicted by the JASPAR database. IÂ ChIP assay verified that ELK1 could bind to GPX4 promoter in Ishikawa cells. JÂ The length of the qPCR product was tested by DNA gelBack to article page