Fig. 6

Establishment, prognostic and clinical validation of the IEscore in LGGs. A Alluvial diagram of Clusters in group with distinct molecular features, IE-Clusters, and IEscore subgroups. B Spearman correlation analysis between IEscore and well-established biological signatures. C The ROC curves represented the prognostic predictive of the IEscore in the three LGG cohorts. D-F Kaplan–Meier curves indicated that higher IEscore LGG patients showed poorer prognosis compared with lower-IEscore LGG patients in the TCGA (D, High-IEscore = 92, Low-IEscore = 359), meta-CGGA (E, High-IEscore = 179, Low-IEscore = 372) and met-GEO (F, High-IEscore = 99, Low-IEscore = 282) LGG cohorts. G-I The violin plots indicated that the distinct IEscore levels among the IDH/1p19q molecular subtypes in the TCGA (G), meta-CGGA (H) and met-GEO (I) LGG cohorts. J The tumor mutation burden (TMB) level was compared between low-IEscore and high-IEscore LGG subgroups. K-L The top frequent mutation genes in high-IEscore LGG patients (K, n = 92) and low-IEscore LGG patients (L, n = 351). The labelled asterisk indicated the statistical p value (* p < 0.05, **p < 0.01 and ***p < 0.001)