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Fig. 9 | BMC Cancer

Fig. 9

From: Construction of a novel model based on cell-in-cell-related genes and validation of KRT7 as a biomarker for predicting survival and immune microenvironment in pancreatic cancer

Fig. 9

Identification of the correlation between KRT7 expression and CIC formation in PC cell lines. A KRT7 expression levels among 15 PC cell lines in three independent datasets. B The expression levels of KRT7 mRNA and protein in BxPC-3, CFPAC-1, PANC-1 and MIA PaCa-2 cell lines. C The frequencies of CIC formation in four cell lines. D Representative immunofluorescent images of typical CIC structures for BxPC-3 and CFPAC-1 cells. Red arrows indicate CIC structures. Scale bar: 100 μm (left) and 10 μm (right). E Related networks and functions of KRT7 predicted by GeneMANIA website. F Representative immunofluorescent images of typical CIC structures for BxPC-3 and CFPAC-1 cells transfected with siRNA of KRT7 or negative control siRNA. Yellow arrows indicate CIC structures. Scale bar: 100 μm (top) and 10 μm (bottom). G The frequencies of CIC formation and cell cluster for KRT7-knockdown BxPC-3 and CFPAC-1 cells. Data represent means ± SD from three independent experiments. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

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