Fig. 5
OL changed the levels of EMT-associated proteins and signaling pathways involved in TGF-β1-mediated invasion and metastasis in Tu686 cells. A Western blotting assay results about the E-cadherin and Vimentin expression in different groups (control, TGF-β1 and TGF-β1 + OL groups). B Western blotting results of Snail and MMP9 in different groups. Western blotting results showing that C TGF-β1 and OL affected the classical Smad2 signaling pathway, D HIF-1α expression, and E pVHL-dependent pathway and PI3K/AKT signaling proteins