Table 1 Primer sequences used for Real-time RT PCR
genes | Forward sequence (5’ − 3’) | Reverse sequence (5’ − 3’) |
|---|---|---|
Bax | TCAGGATGCGTCCACCAAGAAG | TGTGTCCACGGCGGCAATCATC |
Bcl-2 | ATCGCCCTGTGGATGACTGAGT | GCCAGGAGAAATCAAACAGAGGC |
cIAP | CAGACACATGCAGCTCGAATGAG | CACCTCAAGCCACCATCACAAC |
xIAP | TGGCAGATTATGAAGCACGGATC | AGTTAGCCCTCCTCCACAGTGA |
E-cad | GCCTCCTGAAAAGAGAGTGGAAG | TGGCAGTGTCTCTCCAAATCCG |
MMP-9 | GCCACTACTGTGCCTTTGAGTC | CCCTCAGAGAATCGCCAGTACT |
VEGF | TTGCCTTGCTGCTCTACCTCCA | GATGGCAGTAGCTGCGCTGATA |
β-actin | GATAGCACAGCCTGGATAGCA | ACTGGGACGACATGGAGAAAA |