Fig. 5

The effect of SSE on activation of the NF-κB pathway. A The cells were treated with different concentrations of SSE for 6 h, the protein expression levels of p65 and IκBα were analyzed using specific antibodies by western blot. B-C The cells were treated with 10 μM NAC, 10 μM SSE, or 10 μM NAC and 10 μM SSE for 6 h, the p65 subunit translocation from the cytoplasm to the nucleus was evaluated by immunofluorescence, green spots represent p-p65 staining and blue spots represent the cell nuclei (B). The images shown are representatives (Scale bar = 100 μm). The protein expression levels of p65 and IκBα were analyzed using specific antibodies by western blot (C). The black bars and white bars denote 786-O cells and ACHN cells respectively. Data are presented as means ± SD (n ≥ 3). *P < 0.05 and **P < 0.01