Fig. 4From: Sodium selenite inhibits proliferation and metastasis through ROS‐mediated NF‐κB signaling in renal cell carcinomaSSE induced apoptosis and inhibited migration by increasing ROS levels. Following cells were incubated with different concentration of SSE for 6 h, the intracellular ROS levels (A) and mitochondrial membrane potential (B) were determined by fluorospectro photometer. C-E The cells were treated with 10 μM NAC, 10 μM SSE, or 10 μM NAC and 10 μM SSE for 6 h, the intracellular ROS levels were determined by fluorospectro photometer (C), cell migration was determined by wound healing assay (D), and cell apoptosis was determined by flow cytometry (E). The black bars and white bars denote 786-O cells and ACHN cells respectively. Data are presented as means ± SD (n ≥ 3). *P < 0.05 and **P < 0.01Back to article page