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Fig. 7 | BMC Cancer

Fig. 7

From: LAP3 contributes to IFN-γ-induced arginine depletion and malignant transformation of bovine mammary epithelial cells

Fig. 7

IFN-γ upregulated LAP3 by regulation of p38 and ERK MAPKs in BMECs. a-h BMECs were treated with either IFN-γ alone or co-incubated with p38 and ERK inhibitor for 24 h. The expression (a-f) and enzymatic activity (g, h) of LAP3 was detected by western blotting (a, d), qRT-PCR (b, e), and ELISA (c, f-h), respectively. For western blotting, the relative level of the target protein was estimated using densitometry and the ratio was calculated relative to the GAPDH control. Full-length blots are presented in Supplementary Fig. S16 (a) and S17 (d). Bars represent mean values with error bars to represent SD from three independent replicates. Differences between mean values were assessed by two-tailed Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001, compared to control

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