Fig. 5

Knock-down of HDAC2 abolished the acceleration of cell cycle induced by LAP3 upregulation in BMECs. a Effect of LAP3 inhibitor bestatin on LAP3 and HDAC2 protein expressions in malignant BMECs. Full-length blots are presented in Supplementary Fig. S11. b Effect of HDAC2 inhibitor VPA on HDAC2 and LAP3 protein expressions in malignant BMECs. Full-length blots are presented in Supplementary Fig. S12. c Effect of LAP3 overexpression on LAP3 and HDAC2 protein expressions in normal BMECs. VC, pLenti-CMV-GFP-Puro. pLAP3, pLenti-CMV-LAP3-GFP-Puro. Full-length blots are presented in Supplementary Fig. S13. d Effect of HDAC2 inhibitor VPA on cell cycle proteins cyclin A1, cyclin D1, and p27 expressions in malignant BMECs. Full-length blots are presented in Supplementary Fig. S14. The relative level of the target protein was estimated using densitometry and the ratio was calculated relative to the GAPDH control. Bars represent mean values with error bars to represent SD from three independent replicates. Differences between mean values were assessed by two-tailed Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001, compared to control