Fig. 4

Proliferation and migration effects of cytokines secreted by adipose tissues and cells on TNBC cell lines. A Cell viability analysis was detected in MDA-MB-157, MDA-MB-231, MDA-MB-453 and HCC38 cells incubated with adipose secretion from nonobese and obese TNBC patients for 24 h. n = 5. Statistics based on the unpaired t test. ***p < 0.001. B Migration of MDA-MB-157, MDA-MB-231, MDA-MB-453 and HCC38 cells treated with adipose secretion from nonobese and obese TNBC patients for 24 h were detected using a Transwell coculture method. n = 5. Statistics based on the unpaired t test. *p < 0.05, **p < 0.01. C-D Proliferation (C) and migration (D) of MDA-MB-157, MDA-MB-231, MDA-MB-453 and HCC38 cells co-cultured with normal or insulin-resistant 3T3-L1 adipocytes were examined using a Transwell coculture method. n = 5. Statistics based on the unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001. (E-F) Proliferation (E) and migration (F) of different TNBC cell lines with different treatments. Treatment 1 was the cells co-incubated with 9 µg/ml adiponectin, 10 ng/ml IL-6 and 17 ng/ml TNF-α; treatment 2 was the cells co-incubated with 5 µg/ml adiponectin, 14 ng/ml IL-6 and 20 ng/ml TNF-α. n = 5. Statistics based on the unpaired t test. *p < 0.05, **p < 0.01