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Fig. 3 | BMC Cancer

Fig. 3

From: The acidic tumor microenvironment enhances PD-L1 expression via activation of STAT3 in MDA-MB-231 breast cancer cells

Fig. 3

Acidosis-induced PD-L1 expression is attenuated by targeting the acidic pH of MDA-MB-231 cells. A–C MDA-MB-231 cells were incubated with HCl-treated acidic medium for 18 h; the acidic culture medium was replaced with fresh medium, or NaHCO3 was added for 24 h. Protein levels were analyzed by western blotting (A), mRNA levels were analyzed by qPCR (B), and the culture medium pH was measured using a pH meter (C). D–F MDA-MB-231 cells were incubated with lactic acid-treated acidic medium for 24 h; the acidic culture medium was replaced with fresh medium or NaHCO3 was added for 24 h. Protein levels were analyzed by western blotting (D), mRNA levels were analyzed by qPCR (E), and the culture medium pH was measured using a pH meter (F)

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