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Fig. 2 | BMC Cancer

Fig. 2

From: The acidic tumor microenvironment enhances PD-L1 expression via activation of STAT3 in MDA-MB-231 breast cancer cells

Fig. 2

Tumor-derived lactic acidosis increases PD-L1 expression in MDA-MB-231 cells. A Schematic diagram of the glycolysis pathway in cancer cells. B Summary of the process through which various conditioned media were prepared (CON, control; fresh medium), (NCM, normoxic conditioned medium), (HCM, hypoxic conditioned medium). C–E MDA-MB-231 cells were treated with various conditioned media with/without NaOH for 18 h. Protein levels were analyzed by western blotting (C), mRNA levels were analyzed by qPCR (D), and the culture medium pH was measured using a pH meter (E). F–H MDA-MB-231 cells were treated with lactic acid or sodium lactate, with/without, NaOH for 24 h. Protein levels were analyzed by western blotting (F), mRNA levels were analyzed by qPCR (G), and the culture medium pH was measured using a pH meter (H)

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BMC Cancer

ISSN: 1471-2407

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