Table 2 The concentration of amino acids in the culture supernatant of HCT 116 cells treated with SLC6A14-siRNA or pharmacological blockade with α-MT for 24 h by LC-MS/MS (μmol/L)
From: Blockade of the amino acid transporter SLC6A14 suppresses tumor growth in colorectal Cancer
Amino acid | siNC | si-SLC6A14–981 | si-SLC6A14–1702 | α-MT | F value | P value |
|---|---|---|---|---|---|---|
L-Arg | 980.0 ± 50.0 | 1100.0 ± 70.0 | 1230.0 ± 80.0 | 1250.0 ± 60.0 | 10.900 | 0.003* |
L-Lys | 95.4 ± 10.2 | 145.0 ± 11.3 | 167.0 ± 11.3 | 168.0 ± 14.2 | 24.720 | <0.001** |
L-His | 79.3 ± 8.9 | 78.7 ± 8.7 | 78.4 ± 9.2 | 80.2 ± 12.3 | 0.019 | 0.996 |
L-Orn | 29.6 ± 3.5 | 39.8 ± 4.7 | 37.9 ± 4.2 | 37.0 ± 5.5 | 2.918 | 0.100 |
L-Ala | 147.0 ± 13.0 | 183.0 ± 15.0 | 223.0 ± 16.0 | 230.0 ± 21.0 | 16.330 | <0.001** |
L-Pro | 162.0 ± 13.0 | 187.0 ± 15.0 | 208.0 ± 17.0 | 232.0 ± 24.0 | 8.485 | 0.007* |
L-Thr | 86.1 ± 8.5 | 132.0 ± 11.2 | 127.0 ± 12.2 | 129.0 ± 9.4 | 13.010 | 0.002* |
L-Cit | 5.4 ± 1.2 | 5.5 ± 1.3 | 6.1 ± 1.4 | 5.9 ± 2.6 | 0.125 | 0.943 |
Gly | 182.0 ± 35.0 | 332.0 ± 46.0 | 270.0 ± 48.0 | 338.0 ± 34.0 | 9.276 | 0.006* |
L-Ser | 2.7 ± 1.5 | 17.4 ± 3.4 | 35.7 ± 4.2 | 23.4 ± 3.5 | 51.620 | <0.001** |
L-Asn | 159.0 ± 23.0 | 309.0 ± 35.0 | 326.0 ± 32.0 | 336.0 ± 34.0 | 24.700 | <0.001** |
L-Tyr | 57.9 ± 5.6 | 86.9 ± 8.7 | 102.0 ± 9.2 | 97.4 ± 10.3 | 15.800 | 0.001* |
L-Leu | 117.0 ± 23.0 | 203.0 ± 34.0 | 252.0 ± 25.0 | 268.0 ± 16.0 | 21.550 | <0.001** |
L-Glu | 353.0 ± 28.0 | 432.0 ± 36.0 | 497.0 ± 39.0 | 475.0 ± 42.0 | 9.025 | 0.006* |
L-Ile | 99.3 ± 10.0 | 170.0 ± 12.0 | 185.0 ± 14.0 | 183.0 ± 14.0 | 31.050 | <0.001** |
L-Met | 43.9 ± 5.4 | 57.7 ± 4.6 | 70.2 ± 6.3 | 78.4 ± 8.5 | 16.790 | <0.001** |
L-Trp | 3.0 ± 1.2 | 6.3 ± 1.6 | 9.5 ± 2.4 | 9.5 ± 3.2 | 5.826 | 0.021* |
L-Phe | 47.3 ± 6.8 | 61.1 ± 8.2 | 95.9 ± 10.0 | 83.2 ± 8.2 | 20.310 | <0.001** |
L-Asp | 106.0 ± 25.0 | 148.0 ± 24.0 | 168.0 ± 34.0 | 178.0 ± 38.0 | 3.591 | 0.066 |
L-Val | 65.3 ± 8.2 | 121.0 ± 11.0 | 139.0 ± 12.0 | 142.0 ± 15.0 | 27.260 | <0.001** |