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Fig. 6 | BMC Cancer

Fig. 6

From: Blockade of the amino acid transporter SLC6A14 suppresses tumor growth in colorectal Cancer

Fig. 6

Effects of SLC6A14 on CRC via the activation of mTOR signaling. A The protein expression of mTOR signaling and MMPs in the HCT116 or Caco2 cells transfected with SLC6A14-siRNA or blockade of SLC6A14 with α-MT was analyzed by Western blot. B The relative protein levels of SLC6A14 and mTOR signaling molecules, and β-actin was used as the internal control. C The relative protein levels of MMP2, MMP9, and MT1-MMP, and β-actin was used as the internal control. D Western blot analysis was used to determine the downstream effectors of mTOR in HCT116 or Caco2 cells transfected with SLC6A14-siRNA or blockade of SLC6A14 with α-MT. E The relative protein levels of P-p70S6, P-S6 and P-4EBP1, and β-actin was used as the internal control. F Western blot analysis was used to determine the total-Akt and phosphorylated-Akt (Ser473) in HCT116 or Caco2 cells transfected with SLC6A14-siRNA or blockade of SLC6A14 with α-MT. G The relative protein levels of Akt and P-Akt, and β-actin was used as the internal control. The data are expressed as the mean ± SD of three independent experiments. *P < 0.05

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