Fig. 1

Lyc inhibited HUVECs proliferation in vitro. A Time- and concentration- dependent inhibitory effect of Lyc on HUVECs. HUVECs were treated with Lyc and the cell viability for 24 h and 48 h was analyzed by MTT assay. B The synergetic effect of Lyc and sunitinib in 24 h was also measured by MTT assay. C, D Lyc and/or sunitinib inhibited colony formation significantly on HUVECs. Cells were treated with different concentrations of drugs and the colony formation was analyzed and shown in bar graphs (D). E Flow cytometry analysis. Cells were treated with different concentrations of drugs for 24 h and apoptotic intensity was analyzed by flow cytometry. Data represents mean ± SD, *p < 0.05, n = 3