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Fig. 3 | BMC Cancer

Fig. 3

From: Exosome-derived lnc-HOXB8-1:2 induces tumor-associated macrophage infiltration to promote neuroendocrine differentiated colorectal cancer progression by sponging hsa-miR-6825-5p

Fig. 3

Neuroendocrine differentiated colon cancer cell-derived exosomes enhanced the chemotaxis of TAMs, promoted M2 polarization and upregulated CXCR3 expression. A, Exosomes labeled by PKH67 were absorbed by TAMs when they were cocultured with different exosomes. Scale bar = 20 μm. B, The cocultured of TAMs and CgA Exo resulted in an obvious improvement of migration and invasion ability of TAMs. Scale bar = 200 μm. C, The proportion of M2 macrophages (stained by CD68 and CD206) was determined in TAMs with different treatment by immunofluorescence assay. Scale bar = 50 μm. D-E, qRT–PCR assay (D) and western blot assay (E) exhibited that CXCR3 expression in TAMs treated with CgA Exo was elevated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; TAMs, tumor-associated macrophages; NC, negative control; CgA, chromogranin A; Exo, exosomes

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BMC Cancer

ISSN: 1471-2407

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