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Fig. 2 | BMC Cancer

Fig. 2

From: Exosome-derived lnc-HOXB8-1:2 induces tumor-associated macrophage infiltration to promote neuroendocrine differentiated colorectal cancer progression by sponging hsa-miR-6825-5p

Fig. 2

Neuroendocrine differentiated colon cancer cells secrete exosomes. A, Fluorograms of stable neuroendocrine differentiated colon cancer line (LoVo-CgA) and negative control cell line (LoVo-NC), green fluorescent proteins (GFP) > 95%. Scale bar = 100 μm. B-C, The mRNA expression and protein expression of CgA was determined in LoVo-NC cells and LoVo-CgA cells using qRT–PCR assay (B) and western blot assay (C). D, The exosomes extracted from LoVo-NC cells (NC Exo) and LoVo-CgA cells (CgA Exo) showed typical cup shapes by transmission electron microscopy (TEM). Scale bar = 100 nm. E, The particle size of NC Exo and CgA Exo both ranges from 45 to150nm. F, NC Exo and CgA Exo both expressed exosomal labeled protein CD63, TSG101, Alix and HSPA8. ***, P < 0.001; NC, negative control; CgA, chromogranin A; Exo, exosomes

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