Fig. 2

Cytotoxicity in GBM cells in vitroA-C) After 48 h, TMZ significantly decreased the viability of U87-luc2 cells at 500µM. After 48 and 72 h, TMZ significantly decreased the viability of U251-RedFLuc cells at 500µM (n = 3, 3 replicates). D-F Lapatinib significantly decreased the viability of U87-luc2 and U251-RedFLuc cells at 48 and 72 h (48 h: IC₅₀ = 106 µM and 119 µM; 72 h: IC₅₀ = 4.78 µM and 18.8 µM, respectively; n = 4, 3 replicates). Statistics: Ordinary One-Way ANOVA with Dunnett’s multiple comparisons test (compared to 0 µM: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. Unpaired t-test (U251-RedFLuc vs. U8-luc2; ns, p > 0.05; Mean ± SEM)