Fig. 1
From: Characterization and comparison of human glioblastoma models
Confirmation of Luciferase Transduction in vitro (A) Representative image of a dot blot. Luciferase standard curve 0.5–4 ng. GBM cell lysate (2 µg protein loading, n = 6, 5 technical replicates). B Quantification of luciferase expression. Luciferase expression is not significantly different between U251-RedFLuc cells and U87-luc2 cell lysate samples (p > 0.05; n = 6, 5 technical replicates). C Representative image of in vitro bioluminescence signal of 10,000, 15,000, and 20,000 U87-luc2 or U251-RedFLuc cells 1 min after luciferin exposure (n = 4, 3 technical replicates). D In vitro bioluminescence signal of 10,000 GBM cells for U87-luc2 and U251-RedFLuc. Luciferase activity is significantly higher in U251-RedFLuc cells than U87-luc2 cells (p < 0.0001; n = 4, 3 technical replicates). E In vitro bioluminescence signal of 15,000 GBM cells for U87-luc2 and U251-RedFLuc. Luciferase activity is significantly higher in U251-RedFLuc cells than U87-luc2 cells (p < 0.0001; n = 4, 3 technical replicates). F In vitro bioluminescence signal of 20,000 GBM cells for U87-luc2 and U251-RedFLuc. Luciferase activity is significantly higher in U251-RedFLuc cells than U87-luc2 cells (p < 0.0001; n = 4, 3 technical replicates). Statistics: Unpaired t-test (U251-RedFLuc vs. U8-luc2; Mean ± SEM)