Fig. 3

TTK inhibitors inhibited cell proliferation of GBM cells. A U251 and U87 cells were treated with different concentrations (0, 0.1, 1, 5, 10, 20, 50, 100 nM) of BAY-1217389 (BAY) for 48 h. MTT assay was used to detect the cell viability. B U251 and U87 cells were treated with different concentrations (0, 0.01, 0.1, 0.5, 1, 2, 5, 10 μM) of CFI-402257 (CFI) for 48 h. MTT assay was used to detect the cell viability. C U251 and U87 cells were treated with BAY (U251, 10 and 20 nM; U87, 20 and 50 nM) or CFI (U251, 1 and 2 μM; U87, 2 and 5 μM) for 48 h, western blot was performed to determine the protein levels of cleaved PARP1, cleaved caspase 3, and β-actin. D Quantification of relative protein levels in (C). E Clonogenic assay was performed to assess the colony formation efficiency of U251 and U87 cells with BAY (U251, 5 nM; U87, 10 nM) or CFI (U251, 0.5 μM; U87, 1 μM) treatment. F Quantification of the number of clones in (E). The uncropped blots are displayed in Additional file 1. (Data are mean ± SEM, *p < 0.05, **p < 0.01, n = 3)