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Fig. 2 | BMC Cancer

Fig. 2

From: TTK Protein Kinase promotes temozolomide resistance through inducing autophagy in glioblastoma

Fig. 2

Overexpression of TTK promoted TMZ resistance in GBM cells. U251 and U87 cells were stably transfected with PCMV (Ctr) or PCMV TTK (TTK), then treated with 100 μM temozolomide (TMZ) for 48 and 72 h. A Western blot was used to determine the protein levels of TTK and β-actin. B MTT assay was conducted to analyze the cell viability. C U251 and U87 cells transfected with PCMV (Ctr) or PCMV TTK (TTK) were treated with 100 μM TMZ for 72 h, western blot was performed to determine the protein levels of cleaved PARP1, cleaved caspase 3, and β-actin. D Quantification of relative protein levels in (C). E Clonogenic assay was performed to assess the colony formation efficiency of U251 and U87 cells stably transfected with PCMV (Ctr) or PCMV TTK (TTK) with or without 20 μM TMZ treatment. F Quantification of the number of clones in (E). The uncropped blots are displayed in Additional file 1. (Data are mean ± SEM, #p > 0.05, *p < 0.05, **p < 0.01, n = 3)

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