Fig. 3
From: Tumor specificity of WNT ligands and receptors reveals universal squamous cell carcinoma oncogenes
Squamous epithelium cell carcinoma positive WNTs verification in OSCC. A WNT ligands expression profiling was revealed by RNA-seq analysis in two OSCC patients (GSE70666) [14]. We used the log2 ratio of normalized expression of mRNAs in samples as inputs, calculated the distance using Euclidean distance, and completed the linkage hierarchical clustering method for clustering [15]. Results were visualized by Java Treeview. B qRT-PCR verification the mRNA expression levels of 4 OSCC positive WNTs in adjacent normal and OSCC tissues (Normal: n = 23, OSCC: n = 23, for WNT7B and WNT5A detection; Normal: n = 22, OSCC: n = 22, for WNT2B and WNT10A detection). *: P < 0.05; **: P < 0.01