Fig. 6

Alterations in HSP70’s expression and MAPK signaling by circ_0000045 overexpression in HNSCC cells. A, B Decrease in expression of HSP70 and HSPA8 genes in HNSCC cells caused by circ_0000045’s overexpression. Levels of HSP70 (A) and HSPA8 (B) mRNAs in HNSCC cells were measured by quantitative RT-PCR. C, D Elevations in JNK2 and p38 mRNA levels in FaDu and SCC-9 cells with circ_0000045’s overexpression. Quantitative RT-PCR was conducted to measure JNK2 and P38 mRNA contents in HNSCC cells. E Changes in HSP70, JNK2, and P38 protein abundances in HNSCC cells with elevated circ_0000045’s expression. Western blotting was conducted to detect protein levels in HNSCC cells after transfection for 48 h. However, GAPDH was used as the internal standard. N = 3; Veh: vector; OE: overexpression; HSP70: heat shock 70kDa protein; HSPA8: heat shock 70kDa protein 8; JNK2: c-Jun N-terminal kinase 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; *P < 0.05; **P < 0.01