Fig. 3

Promotion of HNSCC cell proliferation, migration, and invasion through circ_0000045’s overexpression. A Great increase in circ_0000045’s expression in FaDu and SCC-9 cells caused by transfection with overexpressing vectors. The expression of circ_0000045 in HNSCC cells was detected through quantitative RT-PCR using cells transfected with empty vectors as negative controls. B, C Significant elevation in FaDu and SCC-9 cell proliferation rates induced through the overexpression of circ_0000045. The proliferation of FaDu (B) and SCC-9 (C) cells was determined by MTS. D Enhanced invasion capacities of FaDu and SCC-9 cells due to circ_0000045’s overexpression. The invasion of HNSCC cells was assessed through the Transwell system. E High migration speeds of FaDu and SCC-9 cells induced by the overexpression of circ_0000045. The wound-healing assay was conducted to evaluate the migration of HNSCC cells. N = 3; Veh: vector; OE: overexpression; OD490: absorbance at 490 nm; *P < 0.05; **P < 0.01; ***P < 0.001