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Fig. 1 | BMC Cancer

Fig. 1

From: circ_0000045 promotes proliferation, migration, and invasion of head and neck squamous cell carcinomas via regulating HSP70 and MAPK pathway

Fig. 1

Differential expression of circRNAs in HNSCC tissues and cell lines. A A heatmap showing the differential expression of circRNAs between cancerous and adjacent non-cancerous tongue tissue samples, collected from three patients with HNSCC. RNA sequencing was conducted to characterize and quantify circRNA’s expression, after which the circRNA of interest having an elevated expression in HNSCC tissue samples were highlighted in red. B Volcano plots presenting the differential expression of circRNAs in HNSCC tissue samples. The Log10TPM (Carcino/Nor) and Log10P values are shown in the Y and X axes. When circRNA’s expression level is 0, we assumed 0 as 0.000000001 to calculate the value of Log10TPM. C Relative expressional levels of four representative circRNAs in HNSCC tissues. Levels of circRNA expression in HNSCC tissues were assessed using quantitative RT-PCR. N=12 (patient 3-14). D RT-PCR-based validation of circRNA synthesis in HNSCC tissue samples (from patient 3). The identities of circ_0008386, circ_0004949, circ_0000043, and circ_0000045 were confirmed by PCR using divergent primers, genomic DNA, or convergent primers as negative controls. GAPDH was a positive control. E Identification of circRNA junction sites through DNA Sanger sequencing. PCR products from panel D were used for DNA sequencing, after which junction sites were indicated using red triangles. F Relative expressional levels of four representative circRNAs in HNSCC cell lines. Levels of circRNA expression in HNSCC cell lines were assessed using quantitative RT-PCR. N = 3; Nor: adjacent normal tissues; Carcino: HNSCC tissues; cDNA: complementary DNA; gDNA: genomic DNA; GAPDH: glyceraldehyde-3-phosphate dehydrogenase

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