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Fig. 3 | BMC Cancer

Fig. 3

From: Cancer-associated fibroblasts strengthen cell proliferation and EGFR TKIs resistance through aryl hydrocarbon receptor dependent signals in non-small cell lung cancer

Fig. 3

Kyn produced from CAFs mediates the activation of AKT and ERK signals in lung cancer cells. A, Western blotting of phosphorylated AKT, total AKT, and actin in A549 cells treated with PBS, Kyn, and Kyn combined with DMF (Kyn 100 μM, DMF 10 μM, 48 hours). B, Western blotting of phosphorylated ERK1/2, total ERK1/2, and actin in A549 cells treated with PBS, Kyn, and Kyn combined with DMF (Kyn 100 μM, DMF 10 μM, 48 hours). C, the relative cells proliferation of Kyn (100 μM) cultured A549 and Lewis treated with PBS, MK-2206 (10 nM), and Rav (5 nM). D, the cytotoxicity of Kyn (100 μM) cultured A549 treated with PBS, MK-2206 (10 nM), and Rav (5 nM) to Erl (5 μM, 48 hours) and Gef (10 μM, 48 hours). E, the cytotoxicity of Kyn (100 μM) cultured Lewis treated with PBS, MK-2206 (10 nM), and Rav (5 nM) to Erl (5 μM, 48 hours) and Gef (10 μM, 48 hours). * indicates P < 0.05. ** indicates P < 0.01. n.s. indicates no statistical significance

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