Fig. 2

CAFs up-regulated AhR signals in lung cancer cells through Kyn. A, Western blotting of IDO1, TDO2, Kyn in HLF1 and CAFs isolated from lung cancer patients. B, the immunofluorescence of AhR in A549 cells cultured with PBS, HFL1, CAFs, HFL1 combined with Kyn (fibroblast: tumor cell, 1:5, Kyn 100 μM, 48 hours). The scale bar is 10 μm. C, the relative cells proliferation of A549 and Lewis treated with PBS, Kyn (100 μM), and Kyn (100 μM) combined with DMF (10 μM). D, the cytotoxicity of A549 pre-treated with PBS, Kyn, and Kyn combined with DMF (Kyn 100 μM, DMF 10 μM, 48 hours) to Erl (5 μM, 48 hours) and Gef (10 μM, 48 hours). E, the cytotoxicity of Lewis pre-treated with PBS, Kyn, and Kyn combined with DMF (Kyn 100 μM, DMF 10 μM, 48 hours) to Erl (5 μM, 48 hours) and Gef (10 μM, 48 hours). * indicates P < 0.05. ** indicates P < 0.01. n.s. indicates no statistical significance