Fig. 5
In vivo inhibitory effect of CAR-engineered T cells on tumors. A Representative immunofluorescence images of four tumor tissue sections from NOD/SCID xenograft mice injected with CoG133-CAR T cells (sacrificed on day 29 after tumor formation). CD133 is labeled in green, GPC3 is labeled in red and exogenous CD3 is labeled in white; scale bar = 50 μm. B We injected effector cells into NOD/SCID xenograft mice on day 0 and day 7 (arrows marked) after the tumor volume was approximately 100 mm3 and recorded the tumor volume data. C We measured the weight of tumor tissues isolated from the sacrificed mice. D ELISA showing cytokine secretion in mouse blood serum seven days after treatment. All statistics are presented as the means ± SDs. n = 6 per group. * P < 0.001 vs. the normal T, mock T and CD133-CAR T groups; # P < 0.001 vs. the normal T, mock T and GPC3-CAR T groups; § P < 0.001 vs. the normal T, mock T, CD133-CAR T and GPC3-CAR T groups