Fig. 4

Influence on protein expression of death receptors. Relative protein expression analysis of DcR2, DcR3, FADD, TRADD, TNF-R1, and TNF-R2 after treatment with 0.5 µM and 1.5 µM acetylshikonin (AS), shikonin (S), and cyclopropylshikonin (CS) for 24 h in chondrosarcoma cells. Untreated control cells (ctrl) served as reference value and β-actin as loading control (mean ± SD, n = 3). All full-length blots are presented in Supplementary Fig. S1