Fig. 6
Effect of CDC25B overexpression on cellular 5-FU sensitivity and doubling time. CDC25B transfection to UT-SCC-26B cells provided 4 stable transfectants with having different levels of CDC25B overexpression (#5, #6, #12 and #14) (A). The induction of the gene expression caused a significant decrease of IC 50 values of 5-FU in the transfectants, thus sensitizing the cells to 5-FU (B), and significantly shorten the doubling time of each transfectant (C). Western blot analyses and quantification assay on the blots were performed for the target protein and β-actin (A) (See Supplemental Information File 1_Western Blot Raw Data)