Fig. 8

PD-L1 and Claudin-1 expression was positively correlated in FTC. a The expression of Claudin-1 was increased in FTC tissues compared with normal tissues. b The mRNA and protein expression levels of Claudin-1 were examined by RT–qPCR and Western blotting, respectively. c The expression level of Claudin-1 was measured by RT–qPCR and Western blotting after transfection with PD-L1 siRNA in FTC cells. d The mRNA expression levels of Claudin-1 and PD-L1 were measured by RT–qPCR after transfection with miR-199a-5p mimics or miR-199a-5p inhibitor in FTC cells. Data are presented as the mean ± standard error based on at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001