Fig. 7

PD-L1 was a direct target gene of miR-199a-5p. a Schematic diagram of the binding sites of miR-199a-5p in the PD-L1 3′UTR, which was indicated with red characters. b Luciferase reporter assay showed that cotransfection with PD-L1 3′UTR-WT and miR-199a-5p mimics significantly reduced the luciferase activity of the WT reporter vector but not the mutant reporter vector. c The expression level of PD-L1 was measured by RT–qPCR and d western blotting after transfection with miR-199a-5p mimics or miR-199a-5p inhibitor in FTC cells. Data are presented as the mean ± standard error based on at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001