Fig. 3

LncRNA NDRG1 functions as a sponge of miR-96-5p and miR-96-5p is low-expressed in osteosarcoma cells and tissues. A. Nuclear-cytoplasmic fractionation assay results indicated that LncRNA NDRG1 was mainly localized in the cytoplasm of OS cells. The GAPDH and U6 genes were used as cytoplasmic and nuclear controls, respectively. B. Venn diagram showing the overlap of the target miRNAs of NDRG1 predicted by miRcode and miRDB. C. The expression levels of six candidate miRNAs in OS cell lines. U6 was used as internal reference. D. Predicted binding sites of miR-96-5p and LncRNA NDRG1. E. The luciferase activities of the LncRNA NDRG1 luciferase reporter vector (WT or MUT) in MG63 and 143B cells transfected with miR-96-5p mimics or mimics NC. F-G. The expression level of miR-96-5p was measured by qRT- PCR in OS cells and OS tissues. miR-96-5p was down-expressed in OS cells and OS tissues. All data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. (student’s t test)