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Fig. 2 | BMC Cancer

Fig. 2

From: RETRACTED ARTICLE: LncRNA NDRG1 aggravates osteosarcoma progression and regulates the PI3K/AKT pathway by sponging miR-96-5p

Fig. 2

LncRNA NDRG1 promoted proliferation, migration and EMT of osteosarcoma cells in vitro. A. The expression of LncRNA NDRG1 was measured by qRT-PCR in MG63 and 143B cell lines after transfecting LncRNA NDRG1 siRNA. B. The growth curves of OS cells were evaluated by CCK-8 assays in MG63 and 143B cells. C, D. EdU assays was performed to evaluate OS cells proliferation. The samples were imaged at 100× magnification. Scale bar = 100 μm. E, F. Transwell assays were performed to assess the migration ability of OS cells. The samples were imaged at 100× magnification. Scale bar = 100 μm. G, H. The wound healing assays were performed to assess the OS cells migration ability. The samples were imaged at 100× magnification. Scale bar = 100 μm. I, J. The expression of EMT related genes (E-cadherin, N-cadherin and Vimentin) in MG63/143B cells transfected with si-LncRNA NDRG1 or si-NC. All data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. (student’s t test)

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