Fig. 4

TINs were analyzed in tumor tissue from mice treated with PLAG. a Ly6G⁺ neutrophils in tumor tissue from mice treated with PLAG were counted. Among CD45⁺ leukocytes, Ly6G⁺ and CD11b⁺ cells were sorted by FACS. b Ly6G⁺ and CD11b⁺ TINs were analyzed in tumor tissue from mice treated with PLAG. Compared with the tumor only: #P < 0.05, ##P < 0.01, ###P < 0.001; compared with the aPD-L1 only treatment group: $P < 0.05, $$P < 0.01, $$$P < 0.001 (n = 3 for each experiment). N.S, not significant. Mean ± SD. c TINs in tumor tissue from mice treated with PLAG were confirmed by IHC staining with anti-Ly6G and anti-neutrophil elastase. d DAB-positive cells in tumor tissue stained with anti-Ly6G and anti-neutrophil elastase were analyzed. Compared with the tumor only: #P < 0.05, ##P < 0.01, ###P < 0.001; compared with the aPD-L1-only treatment group: $P < 0.05, $$P < 0.01, $$$P < 0.001 (n = 3 for each experiment). N.S, not significant. Mean ± SD