Fig. 3

The reduced lymphocyte population in tumor-bearing mice was restored upon PLAG treatment. a The number of lymphocytes in the PLAG treatment group were counted by CBC. Compared with the negative control: ***P < 0.001; compared with the tumor only: #P < 0.05, ##P < 0.01, ###P < 0.001; compared with the aPD-L1 only treatment group: $P < 0.05 (n = 6 for each experiment). N.S, not significant. Mean ± SD. b The NLR in the blood of tumor-bearing mice treated with PLAG treatment was determined. Compared with the negative control: **P < 0.01; compared with the tumor only: #P < 0.05, ##P < 0.01; compared with the aPD-L1 only treatment group: $P < 0.05 (n = 6 for each experiment). N.S, not significant. Mean ± SD. c T cell populations in the blood of tumor-bearing mice treated with PLAG were evaluated. Among CD3⁺ cells, CD4⁺ cells and CD8⁺ cells were counted by FACS. d, e Blood CD4⁺ and CD8⁺ cells recovered from mice treated with PLAG were analyzed. Compared with the negative control: *P < 0.05, **P < 0.01; compared with the tumor only: #P < 0.05, ##P < 0.01, ###P < 0.001; compared with the aPD-L1-only treatment group: $$P < 0.01, $$$P < 0.001 (n = 3 for each experiment). N.S, not significant. Mean ± SD