Fig. 2

Neutrophils decreased in MB49 tumor-bearing mice treated with PLAG. a The number of neutrophils in the blood of tumor-bearing mice treated with PLAG were analyzed by CBC. Compared with the negative control: ***P < 0.001; compared with the positive control: #P < 0.05, ##P < 0.01; compared with the aPD-L1 only treatment group: $P < 0.05 (n = 6 for each experiment). N.S, not significant. Mean ± SD. b Among CD45⁺ leukocytes, Ly6G⁺ neutrophils were counted in the blood of tumor-bearing mice treated with PLAG + aPD-L1. Ly6G⁺ and CD11b⁺ cells were sorted by FACS. c Ly6G⁺ and CD11b⁺cells were counted in in the blood of tumor-bearing mice treated with PLAG. Compared with the negative control: ***P < 0.001; compared with the tumor only: #P < 0.05, ##P < 0.01, ###P < 0.001; compared with the aPD-L1-only treatment group: $$P < 0.01, $$$P < 0.001 (n = 3 for each experiment). N.S, not significant. Mean ± SD