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Fig. 6 | BMC Cancer

Fig. 6

From: A role for ceruloplasmin in the control of human glioblastoma cell responses to radiation

Fig. 6

DNA damage and expression of DNA repair marker in CP-modulated cells post-IR. Single cell electrophoresis comet assay was performed to measure DNA double-strand breaks in stable transfected U251-MG (A) and U87-MG GB (B) cell lines 2 h after a single exposure to 4 Gy- or 16 Gy-irradiation at 3% oxygen. Tail moment, corresponding to %DNA in tail multiplied by length of tail, is represented in left graph while length of tail is represented in right graph (A and B). Results are expressed as mean ± SEM (n = 7). Then, in same condition, western blotting was performed to evaluate γH2AX expression in U251-MG (C) and U87-MG cells (D) and results are expressed as mean ± SEM (n = 4–7). One-way ANOVA with multiple comparisons (each group was compared to their own normalized control) was performed between Control vs. irradiated cells (*, p-value ≤ 0.05; **, p-value ≤ 0.01; ***, p-value ≤ 0.001; ****, p-value ≤ 0.0001)

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