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Fig. 5 | BMC Cancer

Fig. 5

From: A role for ceruloplasmin in the control of human glioblastoma cell responses to radiation

Fig. 5

Change in superoxide anion production and SOD1 expression in CP-modulated cells post-IR. Superoxide anion production was evaluated by flow cytometry measuring MitoSOX™ fluorescence in stable transfected U251-MG (A) and U87-MG GB (B) cell lines 24 h after a single exposure to 4 Gy- or 16 Gy-irradiation at 3% oxygen. Anion superoxide is expressed as % of positive cells ± SEM (n = 3). In same conditions, western blotting was performed to evaluate SOD1 expression in U251-MG (C) and U87-MG cells (D). SOD1 expression is expressed as mean ± SEM (n = 4). One-way ANOVA with multiple comparisons (each group was compared to their own normalized control) was performed between Control vs. irradiated cells (***, p-value ≤ 0.001; ****, p-value ≤ 0.0001). Two-way ANOVA was performed when cell populations have been compared (#, p-value ≤ 0.05)

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